I have narrowed down to what I believe are the top five most repeated gaming quote. (Although I missed It's a me, Mario!) I'll give you an in depth look at each quote. (Slight spoilers)
"That was too close. You were almost a Jill Sandwich."
Resident Evil is often believed to have the absolute worst voice-acting to ever be in a video game, but one line stood out beyond all. Jill Valentine is stuck within a small room and the roof and is about to fall on her. She is saved by Barry, and as everything goes silent, Barry shouts one of the worst lines we have ever heard, "That was too close. You were almost a Jill Sandwich!" And yes, he does laugh. Jill, who was not surprised, replied with a also strange line, "Haha, you're right!"
"Do a barrel roll!"
Star Fox is a busy guy. He's always getting shot at, but one of the most popular lines comes from Pepper Hare, who would always shout, "Do a barrel roll!", telling you to use a barrel roll to dodge incoming fire. This quote has caught on all around the internet, although I'm not sure why...
"That cake is a lie!"
Portal was filled of memorable lines, but one particular stole the spotlight and is probably the most repeated line of all time from a video game. After steadily being teased with cake, Chell discovers a secret room with many dates and writings on the walls. One of these writings read, "That cake is a lie!" This awkwardly caught on quickly, and is widely considered the most popular quote in a video game.
"But our princess is in another castle."
ARGH! Just when you think you finished the game, you hear from Toad, "But our princess is in another castle!" Super Mario became famous quickly for the line, "It's a me, Mario," but no Nintendo quote have I heard more repeated than this one.
"Would You Kindly?"
From the 2007 game, Bioshock, "Would You Kindly?" is a quote from the mysterious Andrew Ryan. Without spoiling anything, this line is used throughout the entire game relentlessly, in the most bizarre situations. There is a deeper reason it has caught on, best discovered if you play the game yourself.
So there we have it! Which one of these is your favorite?