The game will be available first on Steam and GOG, followed "shortly" by a release on the Humble Store. A price was not mentioned.
The game is currently available on Xbox Live for $15.
Spelunky for PC will launch this summer alongside versions for PlayStation 3 and PlayStation Vita, which were announced in March.
Spelunky first hit the PC in 2009. That version is now referred to as Spelunky Classic and will remain free to download through the developer's website.
For more on Spelunky, check out GameSpot's review.