In the game's multiplayer component, gamers will play as a member of Joker or Bane's gang and attempt to take down Batman and Robin, the source said.
Villains in the game's multiplayer mode reportedly include Killer Croc, Deadshot, Firefly, Black Mask, Deathstroke, and "some kind of Electroman character." Deathstroke was previously confirmed as one of the villains in Batman: Arkham Origins.
No further details were divulged and a Warner Bros. representative was not immediately available to comment.
Warner Bros. most recently brought multiplayer to the Batman universe in 2011's downloadable 6 vs. 6 online shooter Gotham City Impostors for PC and consoles.
Due out October 25, 2013 for the Xbox 360, PlayStation 3, PC, and Wii U, Arkham Origins takes place years before the past Arkham games. It stars a "young, unrefined" Batman and will highlight the Caped Crusader's first encounter with many noted villains, including Deathstroke.